生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
分子量
antigen ~37 kDa
物種活性
mouse, human, rat
濃度
~1.0 mg/mL
技術
immunoprecipitation (IP): 5-10 μg using K562 cell extract
western blot: 0.5-1 μg/mL using K562 cell extract
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... PCBP1(5093)
mouse ... Pcbp1(23983)
rat ... Pcbp1(500242)
一般說明
Heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1)/poly(rC)-binding protein 1 (PCBP1) has five major isoforms, hnRNP-E1-E4 and hnRNP-E2-KL (a splice variant of hnRNP-E2). It is a member of the PCBP family or the triple K homology (KH) domain containing protein family. All isoforms of hnRNPE contain three repeats of the ribonucleoprotein KH domain, designated KH1, KH2 and KH3. hnRNP-E1 and hnRNP-E2 are predominantly nuclear with specific enrichment of hnRNP-E1 in nuclear speckles. Among the hnRNPE family, hnRNP-E1 and hnRNP-E2 share the highest amino acid sequence similarity. PCBP1 is ubiquitously expressed and is located on human chromosome 2p13.3.
特異性
Anti-hnRNP-E1 (C-terminal) (also known as PCBP1/2 and αCP1) specifically recognizes human, mouse, and rat hnRNP-E1.
應用
Anti-hnRNP-E1 (C-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.
生化／生理作用
Heterogeneous nuclear ribonucleoproteins (hnRNPs) are important in biological activities such as transcription, pre-mRNA processing, mRNA translation and turnover. hnRNPE proteins are also implicated in mRNA stabilization, translation enhancement and translation silencing. Heterogeneous nuclear ribonucleoprotein E1 (hnRNP E1)/poly(rC)-binding protein 1 (PCBP1) acts as a tumor-suppressive splicing factor. Upregulation of this gene can repress the tumorigenicity of ovarian cancer, prostate cancer, gastric cancer, colon cancer and thyroid carcinoma cells.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
儲存和穩定性
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Common dysregulated genes in endometriosis and malignancies
Dentillo DB, et al.
Revista Brasileira de Ginecologia e obstetricia : Revista da Federac?o Brasileira das Sociedades de Ginecologia e Obstetricia, 38(5), 253-262 (2016)
PCBP1 inhibits the expression of oncogenic STAT3 isoform by targeting alternative splicing of STAT3 exon 23
Wang X, et al.
International Journal of Biological Sciences, 15(6), 1177-1177 (2019)
PCBP-1 regulates alternative splicing of the CD44 gene and inhibits invasion in human hepatoma cell line HepG2 cells
Zhang T, et al.
Molecular Cancer, 9(1), 1-10 (2010)
Margus Varjak et al.
Journal of virology, 87(18), 10295-10312 (2013-07-19)
Alphavirus replicase complexes are initially formed at the plasma membrane and are subsequently internalized by endocytosis. During the late stages of infection, viral replication organelles are represented by large cytopathic vacuoles, where replicase complexes bind to membranes of endolysosomal origin.
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