QPREST27836

SILu^™PrEST ZCPW2

SILuPrESTs Powered by Atlas Antibodies, buffered aqueous solution

• UNSPSC代码: 12352200

属性

• 重组: expressed in E. coli LysA ArgA BL21(DE3)
• 方案: >80% (SDS-PAGE)
• 表单: buffered aqueous solution
• 分子量: predicted mol wt 26kDa including tags
• 纯化方式: immobilized metal affinity chromatography (IMAC)
• 包装: pkg of 1nmol × 5 vials
• 储存条件: avoid repeated freeze/thaw cycles
• 免疫原序列: TATPDESEEGHGEEINMGEKLSKCSPEAPAGSLFENHYEEDYLVIDGIKLKAGECIEDITNKFKEIDALMSEF
• UniProt登记号: Q504Y3
• 运输: wet ice
• 储存温度: −20°C
• 基因信息: human ... ZCPW2(152098)

说明

一般描述

Lys, Arg ¹³C and ¹⁵N metabolically labeled recombinant human protein fragment

应用

Internal standard in MS-based quantitative proteomics

外形

Heavy proteins are provided in solution of 1M Urea PBS, pH 7.4

制备说明

The protein solution should be gently mixed before use. Optimal concentrations and conditions for each application should be determined by the user.

分析说明

Isotopic Label Incorporation

The SILuPrEST standards are produced using metabolic labeling with heavy isotope labeled (15N, 13C) Lysine and Arginine residues, with greater than 99% isotopic label incorporation.

A purification and quantification tag (QTag) consisting of a hexahistidine (His6) sequence followed by an Albumin Binding Protein (ABP) domain derived from Streptococcal Protein G.

All SILuPrESTs contain an N-terminal His6ABP fusion tag, used for accurate determination of concentration. The fusion tag sequence is:

GSSHHHHHHSSGLVPRGSHMASLAEAKVLANRELDKYGVSDYHKNLINNAKTVEGVKDLQAQVVESAKKARISEATDGLSDFLKSQTPAEDTVKSIELAEAKVLANRELDKYGVSDYYKNLINNAKTVEGVKALIDEILAALPGTFAHYMDPNSSSVDKLAAA.

The specific human protein sequence begins directly after ′VDKLAAA′.

法律信息

This product is licensed under U.S. Patent No. 7,396,688 and foreign counterparts from E. I. du Pont de Nemours and Company. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product for research and development only, including services for a third party for consideration. The buyer cannot sell or otherwise transfer this product, its components or materials made using this product or its components to a third party. Information about licenses for excluded uses is available from: E. I. du Pont de Nemours and Company; Attn: Associate Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax), [email protected]

SILu is a trademark of Sigma-Aldrich Co. LLC

安全信息

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1
• 闪点(°F): Not applicable
• 闪点(°C): Not applicable