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種類
Type VII
形狀
buffered aqueous glycerol solution
比活性
350-600 units/mg protein
分子量
237 kDa
濃度
2.0-20.0 mg/mL
異物活動
lactic dehydrogenase and creatine phosphokinase ≤0.01%
phosphoglucomutase and myokinase ≤0.05%
儲存溫度
2-8°C
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一般說明
Research Area: Cell Signaling
Pyruvate kinase plays a role in regulating cell metabolism. There are four pyruvate kinase isoforms in mammals (PKM1, PKM2, PKR, PKL). Mammalian pyruvate kinase is a tetrameric protein composed of identical subunits, arranged in a dimer-of-dimers configuration. Each monomer contains one active site and consists of three main domains- designated A, B, and C-along with a small N-terminal domain. The M2 isoform of pyruvate kinase (PKM2) supports anabolic metabolism and is expressed in cancer and normal tissue.
Pyruvate kinase plays a role in regulating cell metabolism. There are four pyruvate kinase isoforms in mammals (PKM1, PKM2, PKR, PKL). Mammalian pyruvate kinase is a tetrameric protein composed of identical subunits, arranged in a dimer-of-dimers configuration. Each monomer contains one active site and consists of three main domains- designated A, B, and C-along with a small N-terminal domain. The M2 isoform of pyruvate kinase (PKM2) supports anabolic metabolism and is expressed in cancer and normal tissue.
應用
Sigma 公司的丙酮酸激酶已用于制备 大肠杆菌 培养物的 BL21 CP 菌株的无细胞提取物，用于高产无细胞蛋白表达。该酶通过与洗脱的 DAPk（死亡相关蛋白激酶）（一种结合并激活丙酮酸激酶的丝氨酸/苏氨酸激酶）共同孵育，用于测定丙酮酸激酶活性。
Pyruvate Kinase from rabbit muscle has been used as a component in refolding buffer for malate dehydrogenase (MDH) assay. It has also been used as a supplement in assay buffer for phosphoglycerate mutases (PGM) enzyme assays.
生化／生理作用
丙酮酸激酶催化磷酸基团从磷酸烯醇丙酮酸 (PEP) 转移到 ADP。这种转移产生一分子丙酮酸盐和一分子 ATP。分子量：237 kDa，以分子量为 57 kDa 的四个等亚基的四聚体形式存在。
最佳 pH 值：~7.5
最佳 pH 值：~7.5
分子量：237 kDa，以分子量为 57 kDa 的四个等亚基的四聚体形式存在。
等电点：7.6
最佳 pH：~7.5
最佳温度：25°C
εA 280= 0.541 mg (p)/ml，1 cm 路径
在 pH 7.4 和 30°C 的 Tris 缓冲液中，报告的 K M 值为 ATP (0.86 mM)、丙酮酸盐 (10 mM)、ADP (0.3 mM) 和 PEP (0.07 mM)。丙酮酸激酶对磷酸烯醇式丙酮酸具有高度特异性，但可利用其他三磷酸二核苷酸作为底物替代 ATP，包括 GTP、ITP、dATP、UTP 和 CTP。
等电点：7.6
最佳 pH：~7.5
最佳温度：25°C
εA 280= 0.541 mg (p)/ml，1 cm 路径
在 pH 7.4 和 30°C 的 Tris 缓冲液中，报告的 K M 值为 ATP (0.86 mM)、丙酮酸盐 (10 mM)、ADP (0.3 mM) 和 PEP (0.07 mM)。丙酮酸激酶对磷酸烯醇式丙酮酸具有高度特异性，但可利用其他三磷酸二核苷酸作为底物替代 ATP，包括 GTP、ITP、dATP、UTP 和 CTP。
Pyruvate kinase is an enzyme involved in glycolysis and gluconeogenesis. Product P7768 is from rabbit muscle and is provided in a buffered aqueous glycerol solution and has been used in PGM enzyme assays to determine phosphoglycerate mutase activity. Pyruvate kinase is also used to study pyruvate kinase (PK) deficiency
單位定義
在 37°C、pH 7.6 条件下，1 个单位将每分钟 1.0 μmol 磷酸（烯醇）丙酮酸转化为丙酮酸。
外觀
溶于含 0.01 M 磷酸盐的 50% 甘油的溶液，pH 7.0
分析報告
缩二脲法测定蛋白
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Death-associated protein kinase (DAPk), a multi-domain serine/threonine kinase, regulates numerous cell death mechanisms and harbors tumor suppressor functions. In this study, we report that DAPk directly binds and functionally activates pyruvate kinase M2 (PKM2), a key glycolytic enzyme, which contributes
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