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Merck

MBD0063

Sigma-Aldrich

Vitamin Mixture Solution

Ready-made microbiology growth media supplement

别名:

Vitamin mix

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About This Item

分類程式碼代碼:
41106212
NACRES:
NA.75

品質等級

無菌

0.2 μm filtered

形狀

liquid

包裝

pkg of 10 mL

濃度

100 ×

適合性

suitable for (Media supplement)

運輸包裝

dry ice

儲存溫度

-10 to -25°C

一般說明

Vitamin mixtures are used regularly in promoting growth of bacteria and fungi from different types of microbiomes, such as gut, rumen, soil, water, etc,. The use of vitamin mixtures helps scientists reduce time spent on creating the optimal conditions for microbial growth of a specific bacteria as well as diversified culturing of microbial samples. Different types of bacteria (aerobic, anaerobic, extremophiles, etc.) require different combinations of a variety of vitamins to improve their culturability. These include water-soluble vitamins, such as the B vitamins which are a broad category of small molecules that are important for cell metabolism but otherwise do not necessarily share structural or functional characteristics. In microbial studies the use of sequencing techniques is very common for identification of microorganisms in a certain habitat or niche. However, there is a discrepancy between the diversity that one can identify by culture techniques and by sequencing techniques. While the diversity of bacterial species identified by NGS techniques is very high, the number of culturable bacteria and fungi is much lower. With the growth of microbial research, new efforts to optimize culture-based techniques now allow the cultivation of previously uncultivated microbiome bacteria,.

應用

Vitamins, additives, and mineral supplements are used in special culturomics growth media that mimic the natural environment of bacteria and fungi for high throughput culturing. Vitamin mixture solutions contain nutrients and cofactors necessary to support growth from different types of microbial environments like human/animal microbiome, environmental microbiome, as well as other industries such as health and nutrition that rely on microbial research and diagnostics.

特點和優勢

  • Convenient readymade solution to speed up media preparation
  • Sterile solution
  • Optimal mixture for growth of fastidious bacteria as well as diversified culturing of microbial samples

成分

  • Biotin : 2 mg/L
  • Folic acid:2 mg/L
  • Pyridoxamine-HCl: 10 mg/L
  • Thiamine-HCl x 2 H2O: 5mg/L
  • Riboflavin: 5mg/L
  • Nicotinic acid: 5mg/L
  • D-Ca-pantothenate: 5mg/L
  • Cyanocobalamine:0.1mg/L
  • p-Aminobenzoic acid: 5mg/L
  • Lipoic acid:5mg/L
  • KH2PO4:900mg/L

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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访问文档库

B vitamin acquisition by gut commensal bacteria
PLoS Pathogens, 16(1) (2020)
Advancing gut microbiome research using cultivation
Current Opinion in Microbiology, 27, 127-132 (2015)
Serpentinimonas gen. nov., Serpentinimonas raichei sp. nov., Serpentinimonas barnesii sp. nov. and Serpentinimonas maccroryi sp. nov., hyperalkaliphilic and facultative autotrophic bacteria isolated from terrestrial serpentinizing springs
International Journal of Systematic and Evolutionary Microbiology, 71(8), 004945-004945 (2021)
Environmental electroactive consortia as reusable biosensing element for freshwater toxicity monitoring
New Biotechnology, 55, 36-45 (2020)
A Defined Medium for Cultivation and Exometabolite Profiling of Soil Bacteria
Frontiers in Microbiology, 13, 855331-855331 (2022)

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