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Merck

M7167

Sigma-Aldrich

M2 培养基

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

别名:

Cell Culture M2, Cell Culture Medium, M2 Medium Solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

無菌

sterile-filtered

形狀

liquid

儲存條件

protect from light

技術

cell culture | embryo: suitable

雜質

endotoxin, tested

成分

HEPES: 5.42726 g/L
NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.0106 g/L
sodium pyruvate: 0.0363 g/L

運輸包裝

ambient

儲存溫度

2-8°C

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應用

推荐用于室温下操作小鼠胚胎。M2 和 M16 培养基是植入前期胚胎体外培养的常用培养基。它是一种改良的Krebs-Ringer碳酸氢盐溶液,与Whitten′s培养基非常相似。M16含有丙酮酸和乳酸作为能量来源,因为植入前胚胎不能有效利用葡萄糖。

M2培养基是对M16的进一步改良,用HEPES缓冲液替代一些碳酸氢盐。M2可用于在CO2培养箱外长时间收集和处理胚胎。

品質

溶液中的沉淀不影响本品性能。

重構

添加 0.06g/L 青霉素 G 钾盐和 0.05g/L 硫酸链霉素。

分析報告

该产品经过测试,能够支持单细胞小鼠胚胎发育成扩大的胚泡。对胚泡发育的最低要求是 80%。

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Jane C Fenelon et al.
Biology of reproduction, 97(1), 119-132 (2017-06-24)
Embryonic diapause is a common reproductive strategy amongst mammals, requiring an intimate cross-talk between the endometrium and the blastocyst. To date, the precise molecular signals responsible are unknown in the mouse or any other mammal. Previous studies in the mink
Sarah E M Stephenson et al.
Scientific reports, 8(1), 7528-7528 (2018-05-16)
Mutations in PARK2 (parkin) can result in Parkinson's disease (PD). Parkin shares a bidirectional promoter with parkin coregulated gene (PACRG) and the transcriptional start sites are separated by only ~200 bp. Bidirectionally regulated genes have been shown to function in common
Hongzheng Sun et al.
Biology of reproduction, 100(3), 601-617 (2018-09-29)
Methionine adenosyltransferase II (MAT2A) is essential to the synthesis of S-adenosylmethionine, a major methyl donor, from L-methionine and ATP. Upon fertilization, zygotic genome activation (ZGA) marks the period that transforms the genome from transcriptional quiescence to robust transcriptional activity. During
Maryse Lessard et al.
Scientific reports, 9(1), 13829-13829 (2019-09-27)
The paternal environment is thought to influence sperm quality and future progeny may also be impacted. We hypothesized that prenatal exposure to environmentally-relevant contaminants impairs male reproduction, altering embryo gene expression over multiple generations. Folic acid (FA) can improve sperm
Keisuke Yoshida et al.
Nature communications, 9(1), 3885-3885 (2018-09-27)
The majority of histones are replaced by protamines during spermatogenesis, but small amounts are retained in mammalian spermatozoa. Since nucleosomes in spermatozoa influence epigenetic inheritance, it is important to know how histones are distributed in the sperm genome. Conflicting data

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Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

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