M1320

Anti-MTA1 antibody, Mouse monoclonal

clone MTA1-213, purified from hybridoma cell culture

• 别名: Anti-Metastasis-associated Gene 1, Anti-NuRD-70
• MDL號碼: MFCD07370404
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: MTA1-213, monoclonal
• 形狀: buffered aqueous solution
• 分子量: 75-80 kDa
• 物種活性: mouse, human, canine, rat
• 濃度: ~2 mg/mL
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1-2 μg/mL using HeLa cell nuclear extract
• 同型: IgG1
• UniProt登錄號: Q13330
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MTA1(9112) ; mouse ... Mta1(116870) ; rat ... Mta1(64520)

说明

一般說明

Monoclonal Anti-MTA1 (mouse IgG1 isotype) is derived from the hybridoma MTA1-213 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human MTA1. Metastasis-associated genes (MTAs) comprise a novel gene family with a growing number of members. Currently, there are three known genes encoding for six isoforms, MTA1, MTA1S, MTA-ZG29p, MTA2/MTA1L1, MTA3, and MTA3L. MTA1, also known as NuRD-70, was originally identified in rat metastatic adenocarcinomas as a differentially expressed gene. It encodes a 715 amino acid protein that shares about 70% overall homology to human MTA2 and MTA3 proteins, the C-terminus being more divergent than the N-terminus.

免疫原

synthetic peptide corresponding to amino acids 626-641 of human MTA1.

應用

Monoclonal Anti-MTA1 antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA), immunoblotting, immunoprecipitation and immunocytochemistry.

生化／生理作用

Metastasis associated gene 1 (MTA1) is associated with cancer metastasis. MTA1 interacts with the deacetylases within the nuclear remodeling and deacetylation complexes Mi2/ nucleosome remodelling and histone deacetylation (NuRD) suggesting that it is involved in transcriptional repression. MTA1 interacts with cyclin-dependent kinase-activating kinase (CAK), a component of the transcription factor (TFIIH) regulatory complex and act as a signal transducer to mediate crosstalk between corepressor complexes and the general transcription machinery. MTA1 transcriptionally represses estrogen receptor (ER), having serious implications for the development of an aggressive breast cancer phenotype. MTA1s localizes in the cytoplasm and sequesters ER in the cytoplasm, preventing ligand-induced translocation of ER and stimulating malignant phenotypes.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: nwg
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable