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Merck

GE29-0181-83

HiPrep SP High Performance16/10

Cytiva 29-0181-83, pack of 1 column

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About This Item

分類程式碼代碼:
13111000
NACRES:
NA.56

ligand

sulphopropyl

描述

Ion Exchanger Type (value)

包裝

pack of 1 column

製造商/商標名

Cytiva 29-0181-83

參數

1-5 ml/min flow rate
44 psi

床尺寸

16 mm × 100 mm

柱內徑

16 mm

基質

6% cross-linked agarose

粒徑

24-44 μm

樣品平均尺寸

34 μm

cleaning

3-14

工作範圍

4-13

適合性

suitable for bioprocess medium

一般說明

HiPrep SP HP 16/10 is a strong cation exchanger designed for preparative ion exchange chromatography separations. This 20 mL column is prepacked with SP Sepharose High Performance chromatography resin. The resin is based on rigid, highly cross-linked beaded agarose with a mean bead diameter of 34 µm for high resolution separations. High mechanical strength of the matrix allows for high flow rates, together with outstanding resolution. Capacities vary according to the protein applied and its concentration. Loading capacity will also vary depending on the salt concentration, pH, and temperature at which the protein is applied.

特點和優勢

  • For high-performance preparative scale separations where high efficiency and high resolution are desired.
  • Highly cross-linked agarose provides excellent chemical and physical stability.
  • Slim, modern design allows for easy handling and connection.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiPrep is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

实验方案

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

This page covers the use of Sepharose Fast Flow for purification of proteins.

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from Cytiva.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

相关内容

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

This page covers practical problems that may lead to a non-ideal IEX separation and their solutions.

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