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特點
autoclavable In wet form (pH 7.0) at 120°C for 30 min
wetted part: no
包裝
pkg of 50 ea
製造商/商標名
Cytiva 28-9180-10
參數
flow rate (The beads behave as rigid spheres obeying Darcy′s Law.)
床尺寸
9.7 mm × 28 mm
床體積
2.1 mL
柱內徑
9.7 mm
基質
cross-linked dextran
粒徑
40-120 μm (dry)
55-165 (wet)
工作範圍
2-13
一般說明
PD MiniTrap G-10 columns are prepacked, single-use gravity columns for buffer exchange and cleanup of biological samples, for example peptides, small proteins, and oligosaccharides. PD MiniTrap G-10 gravity columns give fast and simple desalting and buffer exchange without any need for a purification system, PD MiniTrap G-10 columns are prepacked and designed for rapid, convenient sample clean-up of small proteins, peptides, oligo-saccharides and other small biomolecules (>700 Mr).
應用
Protein and peptide sample preparation. Desalting/Buffer Exchange/Cleanup.
特點和優勢
- Easy cleanup of peptides, small proteins, or carbohydrates larger than Mr 700, before downstream analysis such as liquid chromatography, electrophoresis, and MS.
- Efficient removal of contaminants such as salts, dyes, and radioactive labels.
- High desalting capacity.
- Preparation of samples in the volume range 100 to 300 μl.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
法律資訊
MiniTrap is a trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
实验方案
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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