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Merck

GE28-4055-00

GST MultiTrap 4B

Cytiva 28-4055-00

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About This Item

分類程式碼代碼:
12161501
NACRES:
NA.32

ligand

glutathione

材料

polypropylene and polyethylene

製造商/商標名

Cytiva 28-4055-00

包裝

pkg of 4 x 96-well

尺寸

127.8 mm × 85.5 mm × 30.6 mm

基質

4% agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning

4-13

工作範圍

4-13

容量

>25 mg binding capacity (horse liver GST/ml medium)

一般說明

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and rapid parallel purification of glutathione S-transferase (GST) tagged proteins.

應用

GST MultiTrap FF and GST MultiTrap 4B are prepacked, 96-well filter plates for reproducible, high-throughput screening and purification of glutathione S-transferase (GST) tagged proteins.

Purification of up to 0.5 mg of GST-tagged Proteins/well directly from unclarified cell lysate can be achieved using GST MultiTrap filter plates. Direct purification of GST-tagged Proteins from unclarified lysate shortens handling time and minimizes degradation of sensitive target Proteins. The 96-well plate format gives great flexibility, both when working with automated robotic systems and manually using centrifugation or vacuum. Consistent well-to-well and plate-to-plate performance ensures high reproducibility.

GST MultiTrap FF is prepacked with Glutathione Sepharose 4 Fast Flow, while GST MultiTrap 4B is prepacked with Glutathione Sepharose 4B.

特點和優勢

  • 96-well filter plate format for high-throughput screening and parallel purification of GST-tagged* Proteins.
  • Highly reproducible well-to well and plate-to-plate results.
  • High purity in one step, high chemical stability. Load unclarified samples directly to the wells.
  • Wells are prepacked with Glutathione Sepharose media for convenience.
  • Easy and predictable scale-up using prepacked GSTrap FF, GSTPrep FF 16/10, and GSTrap 4B columns.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

GSTPrep is a trademark of Cytiva
GSTrap is a trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.

实验方案

This page how to remove GST tags by enzymatic cleavage using Cytiva products.

This page shows how to solubilize membrane proteins with products from Cytiva.

Glutathione Sepharose® High Performance is recommended for high-resolution purification of GST-tagged proteins, providing sharp peaks and concentrated eluent.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

相关内容

This page shows how to solubilize membrane proteins with products from GE Healthcare.

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