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應用
来自猪胰腺的弹性蛋白酶已被用于UMP合成酶（尿苷-5-′单磷酸合成酶）的蛋白水解裂解。它也可和胶原酶一起用于将兔的胸腔、腹主动脉和内膜中的组织分散到单个细胞中。
来自猪胰腺的弹性蛋白酶已被用于鉴定猪胰腺中的一种新型骨/钙代谢调节因子。来自猪胰腺的弹性蛋白酶还被用于研究中性粒细胞弹性蛋白酶的一种特异性合成抑制剂（ONO-5046）对狗急性出血性胰腺炎病程的影响。
生化／生理作用
弹性蛋白酶是由 240 个氨基酸残基组成的单一多肽链，含有 4 个二硫键。分子量约为 25.9 kDa。这种酶被合成为无活性的酶原、弹性蛋白酶原，后者通过胰蛋白酶在 N 末端的有限蛋白水解转化为活性形式。它是一种丝氨酸蛋白酶，具有广泛的特异性。它在小疏水氨基酸（如 Ile、Gly、Ala、Ser、Val 和 Leu ）的羧基侧切割蛋白质。该酶还水解酰胺类及酯类，如 N-苯甲酰-L-丙氨酸甲酯。 pH 最佳值为 8.0-8.5 。它不需要任何激活剂，但可由二异丙基氟磷酸盐、苯甲磺酰氟、α2-巨球蛋白，α1-抗胰蛋白酶、磺酰氟、对二硝基苯基二乙磷酸盐和高盐浓度抑制。弹性蛋白酶广泛用于组织和细胞分离程序。对分离II型肺细胞有效。
弹性蛋白酶水解弹性蛋白（弹性纤维的特定蛋白质）并消化血红蛋白、酪蛋白和纤维蛋白。
包裝
包装规格取决于蛋白质含量
品質
含胰蛋白酶活性
單位定義
一个单元将在 25℃ 下，每分钟，pH8.0，水解 1.0 μmole 的 N-琥珀酰-L-Ala-Ala-Ala-对硝基苯胺。
外觀
含有碳酸钠。
準備報告
2x 结晶和色谱纯化
基底
應用
訊號詞
Danger
危險分類
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
標靶器官
Respiratory system
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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J H Campbell et al.
Arteriosclerosis (Dallas, Tex.), 5(4), 318-328 (1985-07-01)
Smooth muscle cells of the rabbit aorta, when grown in vitro, express three distinguishable forms of phenotype (contractile, reversible synthetic, and irreversible synthetic). We compared the interactions of these three smooth muscle phenotypes with rabbit very low density lipoprotein (VLDL)
R H Kelly et al.
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Induction of acute hemorrhagic pancreatic necrosis by dietary means in mice produces alterations in the serum complement system. Total hemolytic complement, i.e., CH50, and native C3 levels fall during the development of pancreatitis while, at the same time, what could
E E Floyd et al.
The Journal of biological chemistry, 260(16), 9443-9451 (1985-08-05)
The multifunctional protein uridine 5'-monophosphate (UMP) synthase catalyzes the final two reactions of the de novo biosynthesis of UMP in mammalian cells by the sequential action of orotate phosphoribosyltransferase (EC 2.4.2.10) and orotidine 5'-monophosphate (OMP) decarboxylase (EC 4.1.1.23). This protein
Ting Wang et al.
Biochemical and biophysical research communications, 485(2), 284-289 (2017-02-23)
Acute lung injury (ALI) is an early pathophysiologic change in acute respiratory distress syndrome and its management can be challenging. Omalizumab (Xolair™) is a recombinant DNA-derived, humanized antibody. OMZ-SPT is a polypeptide on the heavy chain of omalizumab monoclonal antibody.
Domenico M Guarascio et al.
The Journal of physiology, 599(15), 3697-3714 (2021-06-06)
Taste transduction occurs in taste buds in the tongue epithelium. The Ca2+ -activated Cl- channels TMEM16A and TMEM16B play relevant physiological roles in several sensory systems. Here, we report that TMEM16A, but not TMEM16B, is expressed in the apical part
实验方案
Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.
Chromatograms
application for HPLC我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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