Lu2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: Mouse x Mouse Hybridoma
• 包装: tube of 5 μg 99043003-DNA-5UG; pkg of vial of cells 99043003-1VL
• ImmunoDon: BALB/c x C57 Bl/6
• 骨髓瘤: P3x63Ag8.653
• 反应性: Anti-DR11
• 技术: cell culture | mammalian: suitable
• 同位素/亚型: IgM
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse x Mouse hybridoma

细胞系描述

The cell line Lu2 has been established by fusion of the myeloma P3x63Ag8.653 and spleen cells from BALB/c x C57Bl/6 female mice. Mice received a single i.p. injection with the B cell line LuMar expressing HLA A2.3/B18.5/C5/DR3,11.and B. pertussis three days before fusion. The cell line has been eradicated from mycoplasma using Cibrobay.

免疫原

B cell line LuMar

应用

Immunofluorescence, cytotoxicity, immunoprecipitation

培养基

RPMI 1640 + 2mM glutamine + 10% FBS

传代培养常规

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.