BHL-89

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: human lymph (axillary)
• 包装: tube of 5 μg 98070109-DNA-5UG; pkg of vial of cells 98070109-1VL
• 生长模式: Suspension
• 核型: Hyperdiploid, modal no. 47, chromosome 1 trisomy, 15 monosomy, +14q+
• 形态学: Lymphoblastoid
• 产品: Not specified
• 受体: sIgM,CD22,BB1,HLA-ABC,GnRH
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human B-type EBNA negative lymphoma

细胞系描述

The cell line BHL-89 was established from a 56 year old female patient with chronic lymphoid leukaemia (CLL). The B-cell type cells were shown to express CD9, CD22, CD71,HLA-ABC, BB1, GnRH and surface IgM. Cloning efficiency in soft agar was found to be between 20-60% and population doubling time was 48 hours. BHL-89 cells are Epstein-Barr nuclear antigen (EBNA) negative. They have been shown to be tumourigenic in immunosuppressed mice, sensitive to dibromodulcitol and resistant to the phorbol ester TPA. The cell line was found to be positive for BCL-2 protein and produces small amounts of interferon. Lymphoblastoid cells and floating aggregates. The cell line has been eradicated from mycoplasma at ECACC.

应用

B lymphoma studies, cancer treatments

培养基

RPMI1640 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 1-5 x 100,000 cells/ml;5% CO₂; 37°C

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.