293-PERV-PK-CIRCE

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: human kidney
• 包装: tube of 5 μg 97051411-DNA-5UG; pkg of vial of cells 97051411-1VL
• 生长模式: Adherent aggregates
• 核型: Not specified
• 形态学: Not specified
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human embryo kidney, infected with endogenous retrovirus of pigs

细胞系描述

293-PERV-PK-CIRCE has been established by infecting the human kidney 293 cell line (Sigma Catalogue number. 85120602) with retrovirus produced by the pig kidney cell line PK-15. The endogenous, C-type retrovirus particles produced by PK-15 have been shown to be capable of infection, replication and serial transfer. 293-PERV-PK-CIRCE is useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation. The cell line has tested negative for Hog Cholera virus. Handle under ACDP Category 2 conditions.

应用

Pig retrovirus studies, infectivity assays

培养基

DMEM + 2mM Glutamine + 10% HI-FBS

传代培养常规

Split sub-confluent cultures (70-80%) 1:6 cells can be dislodged by mechanical agitation 5% CO₂ 3

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.