CRE BAG2

95082219, mouse unknown/unspecified, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: CRE BAG2, 95082219
• 生物源: mouse unknown/unspecified
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse NIH 3T3 fibroblast, Moloney murine leukaemia virus transfected

細胞系描述

CRE BAG2 has been established from the NIH 3T3 fibroblast cell line. Cells were transfected with Moloney murine leukaemia virus-derived proviral genomes carrying complementary mutations in the gag-pol or env regions. CRE BAG2 produces BAG ( a β-gal-transducing vector) and can be used for the package of vectors derived from murine leukaemia viruses. The production of recombinant replication-competent helper viruses has never been reported.

應用

Virus-packaging assays, cell-lineage marking systems

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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