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生物来源
human nerve (bone marrow metastasis)
包装
tube of 5 μg 94092302-DNA-5UG
pkg of vial of cells 94092302-1VL
生长模式
Adherent
核型
46
形态学
Polygonal
产品
Not specified
受体
Vimentin
技术
cell culture | mammalian: suitable
相关疾病
metastasis
运输
dry ice
储存温度
−196°C
细胞系来源
Human neuroblastoma
细胞系描述
Derived from a female patient with neuroblastoma from the metastasis of the bone marrow. Tumourigenic in nude mice.
应用
Neuroblastoma differentiation studies
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17
CSF1PO: 10,12
D13S317: 9
D16S539: 14
D5S818: 11,12
D7S820: 11,13
THO1: 9.3
TPOX: 11,12
vWA: 16,17
培养基
DMEM or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
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