生物源
Rat x Mouse Hybridoma
ImmunoDon
Rat Lou/MN spleen cells
骨髓瘤
Sp2/0
反應
Anti-Pgp-1, murine homologue of CD44
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Rat Lou/MN spleen x Mouse Sp2/0
細胞系描述
The hybridoma KM703 was produced by the fusion of Sp2/0 mouse myeloma cells with spleen cells from a Lou/MN rat immunised with the bone marrow derived stromal cell clone, BMS2. The monoclonal antibody recognises an epitope on Pgp-1, the murine homologue of CD44. It prevents the growth of lymphoid or myeloid cells in long term bone marrow cultures.
免疫原
BUS 2 cells
應用
Determination of ligand specifities and function of Pgp-1/CD44 molecules.
The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.
培養基
RPMI 1640 + 2mM glutamine + 0.05mM 2-mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS)
例行更新培養
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C
其他說明
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