C0102

94060811

• 分類程式碼代碼: 12352200

属性

• 生物源: human blood
• HRC Panel 號碼: Not specified
• 增長模式: Suspension
• 染色體組型: Not Specified
• 形態學: Lymphoblastoid

说明

細胞系來源

UK Caucasian Female

細胞系描述

A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.

This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.

應用

As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.

培養基

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 20% FBS / FCS (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

例行更新培養

Maintain cultures between 3 x 10⁵ - 2 x 10⁶ cells/ml; 5% CO₂, 37 °C.

其他說明

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.