IPI-1

93100621, Porcine intestine, Fibroblast-like

• 分類程式碼代碼: 41106514

属性

• product name: IPI-1, 93100621
• 生物源: Porcine intestine
• 增長模式: Adherent
• 染色體組型: Heteroploid
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Boar Miniature male ileum, SV40 transformed

細胞系描述

Fibroblast cell line derived from the ileum of an adult histocompatible miniature male boar. Transformed by SV40 plasmid pSV3-neo.

應用

Potential feeder cell line for the recovery of epithelial cells isolated from the small intestine.

培養基

DMEM + 4mM Glutamine + 0.024IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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