NFS-25 C-3

93090713, mouse blood, Lymphoblast

• 分類程式碼代碼: 41106514

属性

• product name: NFS-25 C-3, 93090713
• 生物源: mouse blood
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblast
• 產品: Not specified
• 受體: Ly-17, Lyb-2, Ly-1, Ly-5 (B220)
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse pre-B lymphoblast

細胞系描述

Derived from a spontaneous lymphoma in an NFS.C58v-1 mouse. These pre-B cells are negative for surface Ig, Ia and ThB.

培養基

DMEM + 10% NCTC 109 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1% Sodium Pyruvate (NaP) + 1mM Oxaloacetic acid + 50μM 2-Mercaptoethanol (2ME) + 2μg/ml Transferrin + 0.2IU/ml Insulin + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Immediately after resuscitation a pre-centrifugation step to remove cryoprotectant (DMSO) is recommended i.e. pellet the cells by centrifugation at 100 x g for 5 minutes and resuspend the cell

其他說明

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