BE10-Late

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: rat brain
• 包装: tube of 5 μg 93040120-DNA-5UG; pkg of vial of cells 93040120-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Not specified
• 产品: Plasminogen activator activity
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 相关疾病: cancer
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Rat, BDIX, foetal brain, malignant

细胞系描述

Malignant cells produced on passaging BE10-Intermediate (Sigma Catalogue number. 93040119) beyond passage 40. The cells possess tissue plasminogen activator activity, are tumourigenic in BDIX rats and form colonies in agar.

应用

Study of carcinogenesis, tumourigenicity studies

培养基

DMEM + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:10 to 1:20 i.e. seeding at 1-2x100,000 cells/25cm² using 0.25% trypsin/EDTA; 10-15% CO₂; 37°C.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.