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Key Documents

MOP-8

92111707, mouse embryo, Fibroblast

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About This Item

分類程式碼代碼:
41106514

product name

MOP-8, 92111707

生物源

mouse embryo

增長模式

Adherent

染色體組型

Not specified

形態學

Fibroblast

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mouse, SV40 Polyoma transformed NIH/3T3 cells

細胞系描述

MOP-8 was developed by transforming NIH/3T3 cells with a hybrid transcription unit composed of the SV40 early promoter fused to the early region of polyomavirus. The cells should be handled under laboratory containment level 2.

應用

Suitable host for transfection studies, polyoma virus replication studies

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA, 5% CO2; 37°C.

其他說明

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