CHH-1

92110412, fish cardiovascular , Not specified

• 分類程式碼代碼: 41106514

属性

• product name: CHH-1, 92110412
• 生物源: fish cardiovascular
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Fish Chum heart

細胞系描述

Established from normal heart tissue from a juvenile Onchorhynchus keta. Cells are susceptible to a wide range of fish viruses.

應用

Aquatic viral studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 5% CO₂; 24°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 medi

其他說明

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