J774A.1 Cell Line from mouse

91051511, mouse blood, Not specified

• 别名: J774 A1 Cells, J774.A1 Cells, J774A1 Cells
• 分類程式碼代碼: 41106514

属性

• product name: J774A.1 Cell Line from mouse, 91051511
• 生物源: mouse blood
• 描述: Mouse BALB/c monocyte macrophage
• 增長模式: Semi-adherent
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Interleukin 1 (IL-1), lysozyme
• 受體: Immunoglobulin, complement
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse BALB/c monocyte macrophage

細胞系描述

Derived from a tumour in a female BALB/c mouse. Cell line produces IL-5 and lysozyme.

應用

J774A.1 cell line has been used to test the cytotoxicity of [1,2,3]triazolo[1,5-a]pyridinium salts (having leishmanicidal activity) from triazolopyridines. It has also been used to determine the antitrypanosomatid activity of flavonoid glycosides isolated from Delphinium sps.

Metabolic studies

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Cells are subcultured by harvesting attached and suspension cells. Remove majority of medium and centrifuge to harvest cells growing in suspension. Scrape adherent cells remaining in the flask. Resuspend cell pellet from centrifugation and combine with scraped cells. Seed into new flask at 1:3 to 1:4 split ratio i.e. seeding at 1-3 x 10,000 cells/cm² Incubate at 37°C; 5% CO₂. Please Note :- On receipt growing order cells may be in suspension. The cells and media should be removed from the flask and spun down to form a pellet. This should then be re-suspended in fresh media and a cell count performed. The cells should then be reseeded at 2-4 x 10E4/cm and should begin to show signs of re-attachment within 48 hours.

其他說明

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