104C1

90110524, guinea pig embryo, Not specified

• 分類程式碼代碼: 41106514

属性

• product name: 104C1, 90110524
• 生物源: guinea pig embryo
• 增長模式: Adherent
• 染色體組型: Diploid
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Guinea pig foetal cells transformed

細胞系描述

Derived from a female foetal carcass of a 2/N guinea pig. The cells were treated with benzopyrene for 7 days and a clone selected at the 20th passage. The cells are tumourigenic in guinea pigs.

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5 x10,000 cells/cm³ using 0.25% trypsin; 5% CO₂; 37°C.

其他說明

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