CCD-39Lu

90110512, human lung, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: CCD-39Lu, 90110512
• 生物源: human lung
• 增長模式: Adherent
• 染色體組型: Modal no. 46
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian lung

細胞系描述

Derived from a lung explant of a Caucasian female infant with mildly severe hyaline membrane disease and hypoxia.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,13
D5S818: 11,12
D7S820: 9,12
THO1: 6,9.3
TPOX: 8,11
vWA: 14,17

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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