3A sub E

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 生物来源: human placenta
• 包装: tube of 5 μg 90110508-DNA-5UG; pkg of vial of cells 90110508-1VL
• 生长模式: Adherent
• 核型: Not specified
• 形态学: Not specified
• 产品: Human chorionic gonadotropin (hCG)
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Human placenta (post crisis); SV40 transformed

细胞系描述

The cell line 3A sub E was derived from 3A(tPA-30-1)after the original line started to senesce and went through crisis. 3A subE has an unlimited lifespan and expresses human chorionic gonadotropin (hCG)

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 9,12
D5S818: 11,13
D7S820: 10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,17

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)

传代培养常规

Aspirate medium and add 0.25% trypsin/EDTA; incubate at room temperature until cells detach. Dilute with fresh medium and aliquot into new flasks at a split ratio 1:2. Renew fluid 1-2 times per week. Incubate culture at 37°C, 5% CO₂ for initial 24 hour

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.