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product name
N1-S1, 90011902
生物源
rat liver
增長模式
Adherent
染色體組型
Not specified
形態學
Epithelial
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Rat hepatoma
細胞系描述
Established from the ascitic fluid of a Novikoff hepatoma. The cells can be synchronised for cell cycle studies. N1-S1 has also been used for studies of pyrimidine metabolism, DNA synthesis and methylation and ribosomal RNA maturation.
應用
Cell cycle, metabolic and mutation studies
培養基
Swimm′s S-77 + 2mM Glutamine + 0.1% Pluronic F68 +10% Foetal Bovine Serum (FBS).
例行更新培養
Maintain cultures between 4x10,000 to 1,000,000 cells/ml; 5% CO2; 37°C. Subculture every 3-4 days by centrifugation or medium addition.
其他說明
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