生物来源
Mouse + Human Transfectoma
包装
tube of 5 μg 87080704-DNA-5UG
pkg of vial of cells 87080704-1VL
ImmunoDon
Not applicable
骨髓瘤
J558L
反应性
Anti-NP (4-hydroxy-3 nitrophenacetyl)
技术
cell culture | mammalian: suitable
同位素/亚型
IgG4
运输
dry ice
储存温度
−196°C
细胞系来源
Mouse J558L + Mouse VH gene + Human C (gamma 4) gene
细胞系描述
This human chimeric antibody is directed against the hapten 4-hydroxy-3 nitrophenacetyl. The V lambda of the endogenous light chain of the plasmacytoma line complements the VNP of the transfected heavy chain to yield the specific antibody. Gene marker: gpt.
培养基
DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
传代培养常规
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.
其他说明
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