CV-1

87032605, African green monkey kidney, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: CV-1, from African green monkey kidney, 87032605, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• 生物源: African green monkey kidney
• 增長模式: Adherent
• 染色體組型: 2n = 60, pseudodiploid
• 形態學: Fibroblast
• 受體: Not specified

说明

細胞系來源

Monkey African Green kidney fibroblast

細胞系描述

Used in Rous sarcoma virus (RSV) transformation studies. Demonstrates rapid thymidine kinase (TK) activity following induction of simian, adeno and papovavirus infections. Derived from a male adult African green monkey kidney. Cells grow rapidly and form monolayers of fibroblast-like cells that support the growth of SV40. Chromosome number shifts have been observed at high passage levels.

培養基

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37 °C.

其他說明

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