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Merck

V79-HG04

87020406, hamster lung, Fibroblast-like

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About This Item

分類程式碼代碼:
41106514

product name

V79-HG04, 87020406

生物源

hamster lung

增長模式

Adherent

染色體組型

Aneuploid

形態學

Fibroblast-like

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Hamster Chinese embyro lung fibroblast transformed

細胞系描述

A spontaneously transformed cell line of a primary culture derived from embryonic lung fibroblasts of a Chinese hamster. Thioguanine resistant, HAT sensitive, high cloning efficiency in soft agar and used for HPRT- genotyping selection in HAT.

應用

Cell fusion studies

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

其他說明

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Selection of the right fusion partner cell line is critical to obtain desired attributes of the hybridoma. Explore hybridoma fusion partners cell lines.

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