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product name
MRC-7, 85020203
生物源
human lung (fetal)
增長模式
Adherent
染色體組型
2n = 46, diploid
形態學
Fibroblast
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human foetal lung
細胞系描述
Established from foetal lung tissue. The cells have a finite lifespan and a similar virus susceptibility to the line MRC-5.
應用
Virus studies: rhinoviruses
培養基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他說明
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