A9

85011426, mouse adipose tissue, Fibroblast

• UNSPSC代码: 41106514

属性

• 产品名称: A9, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA., 85011426
• 生物来源: mouse adipose tissue
• 包装: tube of 5 μg 85011426-DNA-5UG; pkg of vial of cells 85011426-1VL
• 生长模式: Adherent
• 核型: Aneuploid
• 形态学: Fibroblast
• 产品: Not specified
• 受体: Not specified
• 技术: cell culture | mammalian: suitable
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Mouse C3H/An areolar and adipose tissue

细胞系描述

Derivative of L929 selected in azaguanine (3μg/ml). Partially resistant to azaguanine. A9 may be heterozygous for the ability to synthesise active inosinic acid phosphorylase.

应用

Metabolic studies

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-5x10,000/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他说明

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