OX-46

13082205, from Mouse x Mouse Hybridoma

• UNSPSC代码: 41106514

属性

• 生物来源: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• 骨髓瘤: NS0/1
• 反应性: Rat CD48
• 技术: cell culture | mammalian: suitable
• 同位素/亚型: IgG2a
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系描述

The hybridoma cell line OX-46 secretes monoclonal IgG1 (kappa) antibodies reactive with rat CD48.

免疫原

Activated rat T cells

应用

Detection of rat CD48 (cross blocks with OX-45).

培养基

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

传代培养常规

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.