JB1

01091808, Reactivity: Anti-Aggregated HFE protein, Human

• 分類程式碼代碼: 41106514

属性

• ImmunoDon: BALB/c B27 female transgenic mouse
• 骨髓瘤: PS-NS1/Ag 4.1
• 反應: Anti- aggregated HFE protein, Human
• 技術: cell culture | mammalian: suitable
• 同型: IgG3
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Anti-recombinant HFE Protein hybridoma

細胞系描述

To produce this hybridoma mice transgenic HLA-B27 and human β-2 microglobulin were immunized with a preparation of HFE made in recombinant bacteria, refolded with human β-2 microglobulin. Characterisation beyond data in B.J. Haematol 103, 931, 1998 shows that JB1 reacts predominantly with aggregated denatured HFE produced in bacteria, but does not bind folded recombinant HFE produced in CHO cells. In comparison with polyclonal sera raised to the C terminus or the α 3 region of HFE JB1 stains frozen sections of the tissues well, but reacts very weakly with cells transfected with the HFE gene in vitro (usually only visible by confocal microscopy). JB1 does not stain paraffin fixed sections and will not work in western blots.

免疫原

Bacterial recombinant HFE protein

培養基

RPMI 1640, 10% FBS, 1% HAT

例行更新培養

Maintain cells at 3-5x100,000 cells/ml which is usually a split ratio of 1:2. Saturation density os 5 x 100,000 to 1 x 1000,000.

其他說明

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