PNV35B

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC代码: 41106514

属性

• 包装: tube of 5 μg 01080924-DNA-5UG; pkg of vial of cells 01080924-1VL
• ImmunoDon: BALB/c mice
• 骨髓瘤: Ag876
• 反应性: Anti-Osteoclasts (human foetal), ECV304 and U937 cells
• 技术: cell culture | mammalian: suitable
• 同位素/亚型: IgM plus IgG
• 运输: dry ice
• 储存温度: −196°C

说明

细胞系来源

Anti-Osteoclastoma Hybridoma

细胞系描述

135B hybridoma was generated by fusing Ag876 myeloma cells with splenic cells of BALB/c mice immunised with tartrate-resistant acid phosphatase (TRAP) isolated from human osteoclastoma tumour. V35B antibody shows reactivity against the monocytic leukemia cell line U937, ECV304 cells and guinea pig tissue.

免疫原

Osteoclastoma tumour

应用

Immunocytochemistry

培养基

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442) + HT Supplement (H0137)

传代培养常规

Allow hybridomas to become 75% confluent prior to splitting. Seed at 3-9 x 100,000 cells/ml 5% CO₂, 37°C,

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.