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特異性
4-氯-1-萘酚是一种适合用于免疫印迹程序的过氧化物酶底物。该底物产生的不溶性最终产物为蓝色，可目视观察。
應用
4-氯-1-萘酚已被用作酶联免疫斑点（ELISpot）试验的底物，用于检测斑点。它还被用作沉淀生色底物，用于免疫印迹后抗原−抗体反应性条带的成像。
數量
每片含 30 mg 基质。
重構
将 1 片片剂溶于 10 mL 甲醇中。向 10 mL 三乙醇胺缓冲盐水 (pH 7.5) 中加入 2 mL 甲醇贮备液。临用前加入 5μl 新鲜 30% 过氧化氢（Sigma 产品编号：H-1009）。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
dust mask type N95 (US), Eyeshields, Gloves
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商品
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
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