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生物源
Porcine heart
形狀
ammonium sulfate suspension
比活性
≥100 units/mg protein
分子量
98 kDa ( 49 kDa monomer)
溶解度
H2O: soluble 1.0 mg/mL, clear
異物活動
isocitrate dehydrogenase and aconitase ≤0.01%
malic dehydrogenase ≤0.1%
儲存溫度
2-8°C
應用
猪心脏来源柠檬酸合酶用于：
- 注入蝾螈卵子，确定受精时，其在卵子激活中的重要性
- 制备反应混合物，确定丙酮酸羧化酶活性
- 检查其是否能够诱导未受精卵中的[Ca22+]增加和卵子激活
生化／生理作用
柠檬酸合成酶催化柠檬酸在辅酶 A 存在下转化为乙酰辅酶 A，释放 H2O和草酰乙酸。该酶分子量为 85 kDa，pI 为 6.1-6.6。可被氟乙酰辅酶 a、棕榈酰辅酶 a 和柠檬酸辅酶 a 抑制。当其被乙酸酐乙酰化或被碘碘化时也受到抑制。
單位定義
一个装置将形成 1.0μ37°C 下 pH 8.0 时每分钟由草酰乙酸和乙酰 CoA 生成的柠檬酸盐摩尔数。
外觀
用 3.2 M(NH4)2SO4 溶液 (pH 7.0) 混悬。
準備報告
溶于水，形成 1 mg/mL 浓度的澄清溶液。
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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商品
Instructions for working with enzymes supplied as ammonium sulfate suspensions
以硫酸铵悬浮液形式提供的酶的使用指南
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