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Merck

C-28057

Sigma-Aldrich

Macrophage Base Medium DXF

Basal Medium, 250 ml

别名:

Macrophage Culture Medium

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

形狀

liquid

包裝

pkg of 250 ml

技術

cell culture | mammalian: suitable

運輸包裝

wet ice

儲存溫度

2-8°C

一般說明

Macrophage Base Medium DXF (supplements sold separately)

應用

The Macrophage Base Medium DXF is the user-customizable version of the Macrophage Generation Media DXF that does not include cytokines. It must be adequately supplemented by the user for the generation of monocyte-derived Macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. In the latter case, the Monocyte Attachment Medium (C-28051) is also needed. Recommended for:Human Mononuclear Cells (hMNC), freshly isolatedHuman Monocytes (hMo), freshly isolated

成分

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

品質

All lots of PromoCell Macrophage Media DXF are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell Macrophage Media DXF is tested for the ability to support Macrophage differentiation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

儲存和穩定性

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

例行更新培養

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儲存類別代碼

10 - Combustible liquids


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商品

Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.

实验方案

使用无血清和无异源细胞培养基在10天内将人外周血单个核细胞(PBMC)体外分化为M1或M2巨噬细胞。欢迎探索我们超350种PromoCell产品。

Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.

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