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形狀
liquid
包裝
pkg of 250 ml
技術
cell culture | mammalian: suitable
運輸包裝
wet ice
一般說明
M1-Macrophage Generation Medium DXF
應用
Macrophage Generation Media have been developed for the efficient generation of monocyte-derived macrophages (MDM) from freshly isolated peripheral blood monocytes or directly from PBMC as a starting material. In the latter case, the Monocyte Attachment Medium (C-28051) is also needed.The M1-Macrophage Generation Medium DXF contains GM-CSF and allows for the generation of M1-polarized macrophages. This xeno-free medium is ready-to-use and includes cytokines for the directed differentiation of M1-like polarized MDM.Recommended for:Human Mononuclear Cells (hMNC), freshly isolatedHuman Monocytes (hMo), freshly isolated
成分
Basal Medium M1 (250ml)SupplementMix M1 (for 250ml)CytokineMix M1: GM-CSF (for 250ml)
品質
All lots of PromoCell Macrophage Media DXF are subjected to comprehensive quality control tests using human peripheral blood mononuclear cells. Each lot of PromoCell Macrophage Media DXF is tested for the ability to support Macrophage differentiation verified by morphological evaluation and flow cytometry analysis. Each lot of PromoCell Monocyte Attachment Medium is tested for its ability to support optimal attachment and viability of peripheral blood Monocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
儲存和穩定性
Store the Basal Medium at 4 to 8°C in the dark, store the SupplementMix at -20°C immediately after arrival. Keep the Cytokine Mix at -20°C for long-term storage or at 4 to 8°C for up to 2 weeks. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the SupplementMix to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Complete Medium supplemented with cytokines should be used within 2 weeks. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.
例行更新培養
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儲存類別代碼
10 - Combustible liquids
商品
Explore over 350 PromoCell products on Sigma.com, including cryopreserved blood cells and cell culture media for various human cells.
实验方案
使用无血清和无异源细胞培养基在10天内将人外周血单个核细胞(PBMC)体外分化为M1或M2巨噬细胞。欢迎探索我们超350种PromoCell产品。
Generate phagocytic M1 and M2 macrophages from human PBMCs in 10 days in serum-free and xeno-free cell culture media. Explore over 350 PromoCell products.
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