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Merck

C-12977

Sigma-Aldrich

Human Mesenchymal Stem Cells (hMSC)

Derived from adipose tissue, 500,000 cryopreserved cells

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About This Item

分類程式碼代碼:
41106514
NACRES:
NA.81

生物源

human adipose tissue

包裝

pkg of 500,000 cells

形態學

(stromal)

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

一般說明

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

細胞系來源

Adipose Tissue

應用

Mesenchymal Stem Cells (MSC), also termed Mesenchymal Stromal Cells, are multipotent cells that can differentiate into a variety of cell types and have the capacity for self renewal. MSC have been shown to differentiate in vitro or in vivo into adipocytes, chondrocytes, osteoblasts, myocytes, neurons, hepatocytes, and pancreatic islet cells. Optimized PromoCell media are available to support both the growth of MSC and their differentiation into several different lineages. Recent experiments suggest that differentiation capabilities into diverse cell types vary between MSC of different origin.Human Mesenchymal Stem Cells (hMSC-AT) are harvested from normal human adipose tissue from individual donors and are provided in a cryopreserved format. The cells are tested for their ability to differentiate in vitro into adipocytes, chondrocytes, and osteoblasts. The cells show a verified marker expression profile that complies with ISCT recommendations, providing well characterized cells (Cytotherapy (2006) Vol. 8, No. 4, 315-317).

品質

Rigid quality control tests are performed for each lot of Mesenchymal Stem Cells. They are tested for cell morphology, proliferation potential, adherence rate, and viability. Furthermore, they are characterized by flow cytometric analysis of a comprehensive panel of markers, namely CD73/CD90/CD105 and CD14/CD19/CD34/CD45/HLA-DR as proposed by the ISCT. Differentiation assays into adipogenic, osteogenic and chrondrogenic are performed for each lot under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, and microbial contaminants (fungi, bacteria, and mycoplasma).

警告

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

例行更新培養

Click here for more information.

其他說明

Recommended Plating Density: 4000 cells per cm2Tested Markers: CD105 positive, CD73 positive, CD90 positive, CD45 negative, CD34 negative, CD14 negative, CD19 negative, HLA-DR negativeDifferentiation capacity to adipogenic, osteogenic and chondrogenic cells

推薦產品

Recommended Primary Cell Culture Media:Link
Recommended Primary Cell Culture Media:Link

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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商品

Are your mesenchymal stem cells really MSCs? Learn more about the MSC characterization guidelines proposed by the International Society for Cellular Therapy (ISCT).

Protocol to differentiate human mesenchymal stem cells into neurons with extensive dendrites and axons in 3 days. Explore over 350 PromoCell products on Sigma.com.

Highly characterized human mesenchymal stem cell lines from bone-marrow, adipose and umbilical cord along with serum-free MSC expansion and differentiation media.

什么是原代细胞?关于动物和人类原代细胞系和培养基的全面概述,以及它们如何应用于生物医学细胞培养应用。相对于细胞系,原代培养物的均一性较低。

查看所有结果

实验方案

Information about mesenchyme, specifically mesenchymal stem cell procotols. Step-by-step cell culture protocols for mesenchymal stem cell (MSC) isolation, expansion and differentiation.

有关间充质的信息,特别是间充质干细胞培养实验方案用于间充质干细胞(MSC)分离、扩增和分化的分步细胞培养方案。

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