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應用
由于在缺乏特定培养基成分(比如氨基酸、嘌呤或嘧啶)的情况下无法生长,营养缺陷型突变菌株的使用很大程度上大决定了酵母质粒的选择。 用含有突变基因的质粒转化,可使转化菌株能够在缺乏所需成分的培养基上生长。 Sigma 公司的′s 酵母营养缺陷型培养基补充剂提供了一种更丰富的培养基,可以提高产量和生长速度,并增加筛选文库或进行基因敲除时转化的成功率。
成分
除非另有说明,否则补充剂包含以下所有成分:
氨基酸:除亮氨酸外的所有标准氨基酸。所有氨基酸的浓度均为 76mg/L。
其他营养物质:腺嘌呤(18 mg/L),肌醇(76 mg/L),对氨基苯甲酸(8 mg/L),尿嘧啶(76 mg / L)。
氨基酸:除亮氨酸外的所有标准氨基酸。所有氨基酸的浓度均为 76mg/L。
其他营养物质:腺嘌呤(18 mg/L),肌醇(76 mg/L),对氨基苯甲酸(8 mg/L),尿嘧啶(76 mg / L)。
其他說明
氨基酸和其他营养素的混合物加入到无氨基酸酵母氮源。
數量
1 l 培养基将补充 1.6 g。
儲存類別代碼
13 - Non Combustible Solids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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酵母因能够快速生长并具有分散的细胞而被认为是用于真核生物研究的模范系统。酵母培养物可采用类似于细菌培养物的技术在液体培养基或琼脂平板上进行生长、维持和保存。
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