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Merck

W1754

Sigma-Aldrich

PCR Reagent, suitable for PCR

别名:

H2O

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About This Item

线性分子式:
H2O
CAS号:
分子量:
18.02
Beilstein:
2050024
EC號碼:
MDL號碼:
分類程式碼代碼:
12191602
PubChem物質ID:
NACRES:
NA.25

product name

水, PCR Reagent

等級

PCR Reagent

蒸汽密度

<1 (vs air)

蒸汽壓力

3 mmHg

無菌

sterile-filtered

形狀

liquid

包裝

vial of 1.5 mL

技術

PCR: suitable

折射率

n20/D 1.34 (lit.)

pH值

5-7

bp

100 °C (lit.)

mp

0 °C (lit.)

密度

1.000 g/mL at 3.98 °C (lit.)

異物活動

DNase, none detected
RNase, none detected

SMILES 字串

O

InChI

1S/H2O/h1H2

InChI 密鑰

XLYOFNOQVPJJNP-UHFFFAOYSA-N

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一般說明

对 PCR 级水进行无菌过滤。它不含核酸外切酶(dna 酶、rna 酶)和核酸内切酶(切口酶),也不含核酸污染。

應用

水已用于:

  • 作为反应混合物的组分和微流控RT-qPCR的稀释剂
  • 作为反应混合物的组分,以扩增真菌(杂色云芝)DNA的产物
  • 作为稀释剂和反应混合物的组分,以扩增cDNA
  • 水已用于在聚合酶链式反应 (PCR) 中补充样品的最终体积

適合性

适用于聚合酶链式反应 (PCR)

其他說明

便于将水产品规格与 水规格表进行比较

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves


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The free-living amoeboflagellate Naegleria fowleri is the causative agent of primary amoebic meningoencephalitis (PAM), a rapidly fatal disease of the central nervous system. In the United States, the disease is generally acquired while swimming and diving in freshwater lakes and
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商品

Small interfering RNAs (siRNAs) are powerful tools for gene expression knockdown, widely used in molecular biology.

实验方案

Protocol describes amplification of DNA through quantitative PCR with SYBR Green. Consistent batch-to-batch performance can be achieved with large numbers of PCR reactions.

Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.

Protocol for high fidelity amplification of long PCR fragments up to 22kb from complex DNA mixtures and up to 40kb from simple DNA mixtures. AccuTaq LA.

Reverse transcription (RT) is the process of converting RNA to cDNA using a reverse transcriptase enzyme and dNTPs.

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