推荐产品
product name
BL21（DE3）化学感受态细胞, for protein expression
生物源
Escherichia coli
等級
for molecular biology
增長模式
adherent or suspension
形態學
rod shaped
技術
microbiological culture: suitable
細胞轉化
competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
運輸包裝
dry ice
儲存溫度
−70°C
相关类别
一般說明
BL21（DE3）化学感受态菌株含有与IPTG诱导型启动子连接的噬菌体T7 RNA聚合酶基因，可用于含有T7启动子的任何表达质粒。
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
基因型
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
應用
该 大肠杆菌 BL21（DE3）菌株已用于：以pET19b作为表达载体，表达tau 2N4R同种型（aa1–441）和截短的tau（aa1–421）。BL21(DE3)化学感受态细胞已被用于protegrin-1 (PG1) 肽的转化和表达。 在 pHis2载体中表达6His-TEVsite-Hs ARF1 (17-181)-Q71L。
特點和優勢
- 转化效率> 1×107 CFU /µg
- 实惠的价格
- 可为日常蛋白表达工作提供卓越价值。
成分
- BL21（DE3）化学感受态细胞
- pUC19转化对照DNA
- 表达恢复培养基
相關產品
产品编号
说明
价格
儲存類別代碼
10 - Combustible liquids
Romana Koszagova et al.
Microbial cell factories, 17(1), 139-139 (2018-09-05)
Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction
David B Sauer et al.
eLife, 9 (2020-09-02)
Citrate, α-ketoglutarate and succinate are TCA cycle intermediates that also play essential roles in metabolic signaling and cellular regulation. These di- and tricarboxylates are imported into the cell by the divalent anion sodium symporter (DASS) family of plasma membrane transporters
Yasaman Damestani et al.
Lasers in surgery and medicine, 48(8), 782-789 (2016-10-19)
The development and feasibility of a novel nanocrystalline yttria-stabilized-zirconia (nc-YSZ) cranial implant has been recently established. The purpose of what we now call "window to the brain (WttB)" implant (or platform), is to improve patient care by providing a technique
A validated antibody panel for the characterization of tau post-translational modifications.
Ebru E, et al.
Mol. Neurodegener., 12(1), 87-87 (2017)
Ibrahim Torktaz et al.
Applied microbiology and biotechnology, 102(19), 8389-8402 (2018-08-04)
The celH gene from Clostridium thermocellum encodes a protein containing 900 residues and three components, including Cel5E, Lic26a, and carbohydrate-binding domains. Cel5E is a member of the glycoside hydrolase-5 family and is a bifunctional xylanase/cellulase enzyme. We targeted a semi-hydrophobic
相关内容
BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.
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