生物源
human peripheral blood
重編程方法
Sendai virus
描述
age (40-44)
製造商／商標名
EBiSC™
性別
female
增長模式
adherent (pluripotent)
技術
cell culture | stem cell: suitable
相關疾病
none
運輸包裝
dry ice
儲存溫度
−196°C
一般說明
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
細胞系來源
Depositor
Klinikum der Universität zu Köln
Klinikum der Universität zu Köln
細胞系描述
Derivation
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
OCT 4 Yes - -
FOXD3 Yes -
NANOG Yes - -
SOX2 Yes -
SSEA-4 Yes - -
TRA1-80 Yes -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 22
Cell line karyotype: No larger chromosomal aberrations to be reported; Chr12: Duplication of telomeric
Image / Result
Primary cell type: Peripheral blood mononuclear cell
Tissue collection year: 2016
Passage number reprogrammed: 0
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Gene list:
KLF4
MYC
POU5F1
SOX2
Is the reprogramming vector detectable: No
Merthods used: pcr
Files showing reprogramming vector expressed or silenced: Image / Result
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Analysis of Undifferentiated Cells
Marker expression:
Marker Expressed Immunostaining RT-PCR FACS Enzymatic Assay Expression Profiles
OCT 4 Yes - -
FOXD3 Yes -
NANOG Yes - -
SOX2 Yes -
SSEA-4 Yes - -
TRA1-80 Yes -
DNMT3b Yes -
Morphology:
Differentiation potency
Ectoderm:
Ectoderm
In vitro directed differentiation
Marker Expressed
Nestin Yes
Morphology:
Endoderm:
Endoderm
In vitro directed differentiation
Marker Expressed
SOX17 Yes
Morphology:
Mesoderm:
Mesoderm
In vitro directed differentiation
Marker Expressed
BRACHYURY Yes
Morphology:
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: Negative
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Passage number: 22
Cell line karyotype: No larger chromosomal aberrations to be reported; Chr12: Duplication of telomeric
Image / Result
聯結
Note: EAUA and CLIP must be completed before order fulfillment
例行更新培養
Surface coating: Vitronectin
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 23
No. Vials banked (pre-EBiSC): 25
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 20%
CO2 concentration: 5%
Culture medium: other
Culture base medium: StemFlex™
Main protein source: -
Passage Number banked (pre-EBiSC): 23
No. Vials banked (pre-EBiSC): 25
法律資訊
EBiSC is a trademark of Fraunhofer-Gesellschaft
StemFlex is a trademark of Life Technologies Corporation
儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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