生物来源
human dermis (fibroblast)
重编程方法
Sendai virus
描述
age (N/A)
制造商/商品名称
EBiSC™
性别
male
生长模式
adherent (pluripotent)
技术
cell culture | stem cell: suitable
相关疾病
Parkinson′s disease
运输
dry ice
储存温度
−196°C
一般描述
Induced pluripotent stem cells (iPSCs) are adult cells that have been reprogrammed to an embryonic stem cell–like state. The cells can replicate indefinitely or, under controlled conditions, can be differentiated into any other cell type such as nerve, heart or liver cells. Medical researchers are able to use iPS cells to test how different patients might respond to new drugs or to analyse how genetic diseases develop.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
The EBiSC stem cell bank is a collection of human iPS cells available to academic and commercial researchers for use in disease modelling and other forms of stem cell research. The initial collection has been generated from a wide range of donors representing specific disease backgrounds and healthy controls. EBiSC has established many routine procedures for collecting, expanding and characterizing human iPS cell lines. The stem cell bank includes iPSC cell lines derived from neurodegenerative diseases (Alzheimer′s Disease, Parkinson′s Disease, Dementia, Motor Neuron Disease (ALS) - and Huntington′s Disease), eye and heart diseases, and lines from healthy control donors for age and sex matching.
细胞系来源
Depositor
StemBANCC
StemBANCC
细胞系描述
Derivation
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Is the reprogramming vector detectable: Unknown
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: -
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Cell line karyotype: No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array
Primary cell type: Fibroblast
Reprogramming method
Vector type: Non-integrating
Vector: Sendai virus
Is the reprogramming vector detectable: Unknown
Xeno free conditions: no
Derived under gmp: no
Available as clinical grade: no
Characterization
Microbiology / Virus Screening
HIV 1: Negative
HIV 2: -
Hepatitis B: Negative
Hepatitis C: Negative
Mycoplasma: Negative
Genotyping
Karyotyping
Cell line karyotype: No abnormalities detected
Karyotyping method: Molecular karyotyping by SNP array
联系
Note: EAUA and CLIP must be completed before order fulfillment
传代培养常规
Surface coating: Matrigel®/Geltrex®
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: mTeSR® 1
Passage method: Enzyme-free cell dissociation / EDTA
O2 concentration: 21%
CO2 concentration: 5%
Culture medium: mTeSR® 1
法律信息
EBiSC is a trademark of Fraunhofer-Gesellschaft
GELTREX is a registered trademark of Life Technologies Corporation
Matrigel is a registered trademark of Corning, Inc.
mTeSR is a registered trademark of WiCell Research Institute, Inc.
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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