生物源
goat
品質等級
抗體表格
affinity isolated antibody
抗體產品種類
secondary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
物種活性
rabbit
存貨情形
available only in USA and Canada
濃度
~1 mg/mL
螢光
λex 511 nm; λem 525 nm in PBS, pH 7.4 (photoactivated)
適合性
corresponds for SDS-PAGE
儲存溫度
−20°C
一般說明
Abberior CAGE 500 is a caged dye which is initially colorless and nonfluorescent. When illuminated with UV light it undergoes a rapid uncaging reaction and releases a highly fluorescent dye which fluoresces at 511 nm.
Abberior CAGE 500 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. It performs very well with Leica GSD microscope and other superresolution microscopes such as Nikon N-Storm, particularly as a 2 Color partner with CAGE 552 or FLIP 565.
Further, after photoactivation, Abberior CAGE 500 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities (e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore).
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (photoactivated, PBS pH 7.4), 299 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, εmax [M-1cm-1]: 50 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λem [nm]: 525 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 590-610
Fluorescence Quantum Yield, λ: 0,85 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (photoactivated, PBS pH 7.4)
Abberior CAGE 500 is designed for single-molecule photoswitching microscopy modes such as PALM, STORM and GSDIM and, in its uncaged form, also performs well in STED microscopy. It performs very well with Leica GSD microscope and other superresolution microscopes such as Nikon N-Storm, particularly as a 2 Color partner with CAGE 552 or FLIP 565.
Further, after photoactivation, Abberior CAGE 500 can be tracked to analyze molecular dynamics such as diffusion, flow directions and velocities (e.g. are tracking experiments in which the fluorescence signal is observed over time after the initial release of the fluorophore).
Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 511 (photoactivated, PBS pH 7.4), 299 (non-photoactivated, PBS pH 7.4)
Extinction Coefficient, εmax [M-1cm-1]: 50 000 (photoactivated, PBS pH 7.4)
Photoactivation Wavelength, λ [nm]: 360-440
Fluorescence Maximum, λem [nm]: 525 (photoactivated, PBS pH 7.4)
Recommended STED Wavelength, λ [nm]: 590-610
Fluorescence Quantum Yield, λ: 0,85 (photoactivated, PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 4,1 (photoactivated, PBS pH 7.4)
適合性
Designed and tested for fluorescent super-resolution microscopy
分析報告
May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence
unconjugated dye ≤5% of total fluorescence
法律資訊
abberior is a registered trademark of Abberior GmbH
未找到合适的产品?
试试我们的产品选型工具.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
American journal of translational research, 11(1), 233-244 (2019-02-23)
Dental stem cell biotechnology has been used as a potential method to treat the dental diseases. This study aimed to investigate effects of mechanical stimulation on osteogenic properties of rat dental mesenchymal stem cells (DMSCs). DMSCs were isolated from rat
Chemistry (Weinheim an der Bergstrasse, Germany), 16(15), 4477-4488 (2010-03-24)
New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, N',N-bis(2,2,2-trifluoroethyl) derivatives 1 e, 1 i, 1 j, 3-H and 5 were found to
Rhodamines NN: a novel class of caged fluorescent dyes.
Angewandte Chemie (International ed. in English), 49(20), 3520-3523 (2010-04-15)
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门