10072801

KF1 cell line

• 分類程式碼代碼: 41106514
• NACRES: NA.81

属性

• 生物源: fish fin
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Koi carp fin cell line, koi herpesvirus isolation

細胞系描述

The KF1 cell line was developed from Koi carp (Cyprinus carpio koi) fin.

DNA分析

Not specified

培養基

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). If a CO₂ incubator is not available, 10 mM HEPES can be included.

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:4, i.e., seeding at 2 x 104 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 25 °C.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable