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Merck

01696

Sigma-Aldrich

丙烯酰胺

for Northern and Southern blotting, powder blend

别名:

2-丙烯酰胺, 丙烯酸酰胺

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About This Item

线性分子式:
CH2=CHCONH2
CAS号:
分子量:
71.08
Beilstein:
605349
EC號碼:
MDL號碼:
分類程式碼代碼:
41105319
eCl@ss:
39031205
PubChem物質ID:
NACRES:
NA.25

等級

for molecular biology

品質等級

蒸汽密度

2.45 (vs air)

蒸汽壓力

0.03 mmHg ( 40 °C)

產品線

BioReagent

化驗

≥99.5% (GC)

形狀

powder blend

技術

electrophoresis: suitable

雜質

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

bp

125 °C/25 mmHg (lit.)

mp

82-86 °C (lit.)

溶解度

H2O: 0.25 g/mL at 20 °C, clear, colorless

儲存溫度

2-8°C

SMILES 字串

NC(=O)C=C

InChI

1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)

InChI 密鑰

HRPVXLWXLXDGHG-UHFFFAOYSA-N

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一般說明

Acrylamide powder is a white, odorless, crystalline solid. It is commonly used in molecular biology labs as a cross linking agent to cast polyacrylamide gels for nucleic acid electrophoresis.

應用

Suitable for making polyacrylamide gels according to established recipes.

注意

Acrylamide powder is highly toxic through inhalation and skin contact. Acrylamide is considered to be a probable carciongen in humans. Standard laboratory nitrile gloves and full sleeve lab coats should be worn when handling acrylamide in any form. Dust masks or working in a fume hood is advised when handling acrylamide powder.

象形圖

Skull and crossbonesHealth hazard

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

標靶器官

Peripheral nervous system

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

280.4 °F - closed cup

閃點(°C)

138 °C - closed cup

個人防護裝備

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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分析证书(COA)

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R.C. Ogden et al.
Methods in Enzymology, 152, 66-66 (1987)
Janneke G F Hogervorst et al.
Carcinogenesis, 35(5), 1032-1038 (2014-01-09)
Acrylamide, a probable human carcinogen, is present in heat-treated carbohydrate-rich foods. Epidemiological studies have not shown a clear association between acrylamide intake and colorectal cancer (CRC) risk. This may be due to the molecular heterogeneity in colorectal tumors, which was
Rusty L Montgomery et al.
EMBO molecular medicine, 6(10), 1347-1356 (2014-09-23)
Over the last decade, great enthusiasm has evolved for microRNA (miRNA) therapeutics. Part of the excitement stems from the fact that a miRNA often regulates numerous related mRNAs. As such, modulation of a single miRNA allows for parallel regulation of
Franco Pedreschi et al.
Journal of the science of food and agriculture, 94(1), 9-20 (2013-08-14)
Acrylamide (AA) is known as a neurotoxin in humans and it is classified as a probable human carcinogen by the International Agency of Research on Cancer. AA is produced as by-product of the Maillard reaction in starchy foods processed at
Arianna Rath et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(39), 15668-15673 (2013-09-11)
SDS/PAGE is universally used in biochemistry, cell biology, and immunology to resolve minute protein amounts readily from tissue and cell extracts. Although molecular weights of water-soluble proteins are reliably determined from their SDS/PAGE mobility, most helical membrane proteins, which comprise

实验方案

Northern和Southern印迹简介:两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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