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Merck

00879

Sigma-Aldrich

8-Acetoxypyrene-1,3,6-trisulfonic acid trisodium salt

for fluorescence, ≥98.0% (TLC)

别名:

1-Acetoxypyrene-3,6,8-trisulfonic acid trisodium salt, Trisodium-8-acetoxypyrene-1,3,6-trisulfonate

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About This Item

经验公式(希尔记法):
C18H9Na3O11S3
分子量:
566.42
Beilstein:
9538267
MDL號碼:
分類程式碼代碼:
12352200
PubChem物質ID:

等級

for fluorescence

化驗

≥98.0% (TLC)

mp

238-248 °C (dec.) (lit.)

溶解度

DMF: soluble
DMSO: soluble
H2O: soluble
methanol: soluble

螢光

λex 460 nm; λem 510 nm in 0.1 M potassium phosphate pH 8.0 (esterase)

儲存溫度

−20°C

SMILES 字串

[Na+].[Na+].[Na+].CC(=O)Oc1cc(c2ccc3c(cc(c4ccc1c2c34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O

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應用

A membrane permeable, nonfluorescent reagent . This fluorochrome enters the cell cytoplasm, where its acetic group is cleaved by intracellular, nonspecific esterases, which forms the impermeable, fluorescent form of the molecule. Utilized as a phloem-mobile symplasmic tracer having staining characteristics that differ from the commonly used CFDA (5-(6-)carboxyfluorescein diacetate).
suitable as fluorogenic substrate for esterases

其他說明

Highly water soluble enzyme substrate for the fluorimetric assay of esterases at longwave excitation and emission wavelengths

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分析证书(COA)

Lot/Batch Number

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O S Wolfbeis et al.
Analytical biochemistry, 129(2), 365-370 (1983-03-01)
A direct and continuous kinetic method for the fluorimetric assay of various hydrolases by using new, highly water-soluble substrates is described. The latter consist of esters of strongly fluorescent 1-hydroxypyren-3,6,8-trisulfonic acid trisodium salt with acetic, butyric, caprylic, and oleic acid.

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