等級
for fluorescence
化驗
≥98.0% (TLC)
mp
238-248 °C (dec.) (lit.)
溶解度
DMF: soluble
DMSO: soluble
H2O: soluble
methanol: soluble
螢光
λex 460 nm; λem 510 nm in 0.1 M potassium phosphate pH 8.0 (esterase)
儲存溫度
−20°C
SMILES 字串
[Na+].[Na+].[Na+].CC(=O)Oc1cc(c2ccc3c(cc(c4ccc1c2c34)S([O-])(=O)=O)S([O-])(=O)=O)S([O-])(=O)=O
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應用
A membrane permeable, nonfluorescent reagent . This fluorochrome enters the cell cytoplasm, where its acetic group is cleaved by intracellular, nonspecific esterases, which forms the impermeable, fluorescent form of the molecule. Utilized as a phloem-mobile symplasmic tracer having staining characteristics that differ from the commonly used CFDA (5-(6-)carboxyfluorescein diacetate).
suitable as fluorogenic substrate for esterases
其他說明
Highly water soluble enzyme substrate for the fluorimetric assay of esterases at longwave excitation and emission wavelengths
Analytical biochemistry, 129(2), 365-370 (1983-03-01)
A direct and continuous kinetic method for the fluorimetric assay of various hydrolases by using new, highly water-soluble substrates is described. The latter consist of esters of strongly fluorescent 1-hydroxypyren-3,6,8-trisulfonic acid trisodium salt with acetic, butyric, caprylic, and oleic acid.
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