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Merck
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PICM0RG50

Millipore

Millicell器官培养型站立式培养小室, 亲水PTFE膜, 0.4 µm, 30 mm, 50/pk

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, size 6 wells, sterile

别名:

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm, cell culture inserts, organotypic inserts, plate inserts

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About This Item

分類程式碼代碼:
41104923
eCl@ss:
32011202
NACRES:
NB.22

材料

polystyrene housing
transparent PTFE membrane

品質等級

400

無菌

ethylene oxide treated
sterile

特點

hydrophilic

製造商／商標名

Millicell®

包裝

pkg of Individually blister packaged

參數

50 °C max. temp.

技術

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

高度

5 mm

直徑

30 mm

尺寸

6 wells

表面積

4.2 cm2

顏色

transparent, when wetted

基質

Biopore™

孔徑

0.4 μm pore size

結合類型

low binding surface

檢測方法

fluorometric

運輸包裝

ambient

相关类别

一般說明

凭借Millicell嵌套板，附着或悬浮细胞可以从其上下两侧接触到培养基。更加密切地模拟了体内所发生的细胞生长、结构和功能。另外，Millicell嵌套板可用于从两侧研究单层细胞。

應用

  • 细胞附着、3D 细胞培养、细胞生长、细胞分化、免疫细胞化学
  • Biopore 膜（亲水PTFE）是低蛋白结合、活细胞观察和免疫荧光应用的理想选择。

特點和優勢

  • 三维外植体结构的高活力和优越性研究
  • 较低的高度令其适合装入标准培养皿
  • Biopore (PTFE) 膜提供高活力—长达40天—和良好的跨膜氧转运
  • 该膜是透明的，并针对器官型外植体的长期维护进行优化。

法律資訊

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

10-13 - German Storage Class 10 to 13

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输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Keisuke Ikegami et al.
Investigative ophthalmology & visual science, 61(3), 26-26 (2020-03-18)
Elevated IOP can cause the development of glaucoma. The circadian rhythm of IOP depends on the dynamics of the aqueous humor and is synchronized with the circadian rhythm pacemaker, that is, the suprachiasmatic nucleus. The suprachiasmatic nucleus resets peripheral clocks
Ryota Tamura et al.
Human gene therapy, 31(5-6), 352-366 (2020-02-23)
Glioblastoma is the most aggressive brain tumor characterized by diffuse infiltration into the normal brain parenchyma. Neural stem cells are known to possess the tumor-tropic migratory capacity and thus can be used as cellular vehicles for targeted delivery of therapeutic
Andrea S Cupp et al.
Biology of reproduction, 68(6), 2033-2037 (2003-02-28)
The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane
R Le Bouffant et al.
Human reproduction (Oxford, England), 25(10), 2579-2590 (2010-07-31)
The initiation of meiosis is crucial to fertility. While extensive studies in rodents have enhanced our understanding of this process, studies in human fetal ovary are lacking. We used RT-PCR and immunohistochemistry to investigate expression of meiotic factors in human
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实验方案

Toluidine Blue Staining Protocol for Millicell® Inserts

Toluidine blue selectively stains nuclear material and acidic tissue components, aiding in histological staining for tissues rich in DNA/RNA.

Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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Basic Indirect Co-culture on Both Sides of Millicell® Cell Culture Insert Membranes

This page covers the basic indirect co-culture procedure on both sides of Millicell cell culture insert membranes.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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